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Identification of changes in the behaviour of tumour cells in low concentrations of migrastatics by coherence-controlled holographic microscope
Zábranská, Magdaléna ; Netíková,, Irena Štenglová (referee) ; Veselý, Pavel (advisor)
The subject of the diploma thesis was using of Coherence-Controlled Holographic Microscope in the research of the effect of migrastatics at different concentrations on tumor cells and the interpretation of quantitative phase imaging data. The theoretical introduction is divided into two parts. The first part is devoted to CCHM, which briefly describes the history of holographic microscopy, the principle and a detailed description of the main parts of the microscope. The second part is an introduction to the issue of cancer treatment and the introduction of migrastatic drugs. The experimental part of the work is the analysis of the influence of migrastatics on two tumor lines (H1299 and HT1080) from the measured time-lapse records taken by a holographic microscope. The effect of drugs was evaluated using characteristics describing morphological and dynamic cell changes in the SophiQ software. The data were then graphically and statistically processed to compare and evaluate the effect of migrastatics at low levels. In conclusion of the work, the findings were summarized and selected characteristics were proposed to analyze the impact of migrastatics.
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Liposomal forms of drugs for the treatment of lung diseases caused by selected types of bacteria
Janoušková, Vendula ; Smilek, Jiří (referee) ; Mravec, Filip (advisor)
This diploma thesis deals with the study of the encapsulation efficiency of the antibiotic doxycycline encapsulated in the individual liposomal systems depending on different composition of the components. We also discussed the kinetics of the encapsulated drug releasing from the carrier systems. The liposomal systems for the treatment of local lung diseases were selected due to their suitable physicochemical properties. The bacterium called Staphylococcus aureus was chosen as a model of the local lung disease, pulmonary pneumonia. Doxycycline was chosen as the antibiotic ideal for the treatment of staphylococcal infection. The aim of the diploma thesis was to optimize the composition of liposomes which would have application potential for the inhalation administration of drugs. Two suitable liposomal systems have been prepared consisting of the addition of various components as cholesterol and phosphatidic acid. We have succeeded in determination of the drug concentration for the encapsulation which had sufficient antimicrobial efficacy to treat the local lung disease. The cytotoxic tests were used to determine the side effects of selected liposomal systems. Furthermore, the individual liposomal systems were characterized in terms of size, zeta potential and kinetic stability which are other important properties effecting their future application potential.
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Identification of changes in the behaviour of tumour cells in low concentrations of migrastatics by coherence-controlled holographic microscope
Zábranská, Magdaléna ; Netíková,, Irena Štenglová (referee) ; Veselý, Pavel (advisor)
The subject of the diploma thesis was using of Coherence-Controlled Holographic Microscope in the research of the effect of migrastatics at different concentrations on tumor cells and the interpretation of quantitative phase imaging data. The theoretical introduction is divided into two parts. The first part is devoted to CCHM, which briefly describes the history of holographic microscopy, the principle and a detailed description of the main parts of the microscope. The second part is an introduction to the issue of cancer treatment and the introduction of migrastatic drugs. The experimental part of the work is the analysis of the influence of migrastatics on two tumor lines (H1299 and HT1080) from the measured time-lapse records taken by a holographic microscope. The effect of drugs was evaluated using characteristics describing morphological and dynamic cell changes in the SophiQ software. The data were then graphically and statistically processed to compare and evaluate the effect of migrastatics at low levels. In conclusion of the work, the findings were summarized and selected characteristics were proposed to analyze the impact of migrastatics.
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Topical and systemic treatment of acne vulgaris
Ackermannová, Veronika ; Hrdina, Radomír (advisor) ; Vopršalová, Marie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Veronika Ackermannová Supervisor: prof. Radomír Hrdina, MD, CSc. Title of diploma thesis: Topic and systemic treatment of acne vulgaris Acne vulgaris is a skin disease affecting the hair follicles and sebaceous glands. The disease manifests itself by increased sebum production, non-inflammatory (comedones) and inflammatory lesions (papules, pustules, nodules, cysts). It occurs predominantly in adolescents, but may persist into adulthood. It is a multifactorial disease, which is caused by several factors (internal and external stimuli). The major pathogenetic factors include increased sebum production, hyperkeratosis, P. acnes colonization and inflammation present. First, it is necessary to diagnose the type of acne in order to choose the right and effective therapy, because there is not only one type of acne. There are many types and variants of acne, and although they show similar symptoms (affecting the follicles of sebaceous glands), their cause often differs. There is no uniform classification system for acne vulgaris and it varies between authors. Some authors classify acne vulgaris according to severity into mild, moderate and severe, others into comedonic, papulopustular, nodulocystic...
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Voltammetric determination of drugs doxycycline and dimenhydrinate using carbon film electrode
Humpolíková, Jiřina ; Fischer, Jan (advisor) ; Dejmková, Hana (referee)
This bachelor's thesis concerns the determination of doxycycline (DX) and dimenhydrinate (DMH) by the methods DC voltammetry (DCV) and differential pulse voltammetry (DPV) on a carbon film electrode (CFE). The optimum conditions for voltammetric determination of DX and DMH were found and under these conditions, concentration dependences were measured and the limits of quantification (LOQ) were calculated for each method. The media of BR-buffer pH 12.0 was chosen as optimum for DCV and DPV determination of DX at CFE. For both DCV and DPV, the linear concentration dependences were obtained in concentration ranges of DX from 20 µmol·l-1 to 100 µmol·l-1 with LOQ 15,4 µmol·l-1 for DCV and 15,2 µmol·l-1 for DPV. DX was determined in drug using DCV under optimized conditions and for comparison of used voltammetric method DX in drug was determined also using UV-VIS spectrophotometry with consistent results. The optimum medium of BR buffer pH 3.0 was chosen for determination of DMH by DCV and pH 2.0 for DPV. The calibration range 2,0 - 10,0 µmol·l-1 of DMH was received using DCV and 0,2 - 1,0 µmol·l-1 of DMH was recevied using DPV at CFE with reached LOQ 0,08 µmol·l-1 for DCV and 0,02 µmol·l-1 for DPV. DMH was determined in comertional drugs using DCV on founded conditions. The stability of stock solution...
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